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ActoFactor™ Recombinant Human KIT ligand (Insect)
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Cat.No.
CSC-CTK0292
Description
Recombinant Human Stem Cell Factor (rHu SCF) is a hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis. rHuu SCF stimulates the proliferation of myeloid, erythroid, and lymphoid progenitors in bone marrow cultures and has been shown to act synergistically with colony stimulating factors. Recombinant Human SCF produced in insect cells is a single, glycosylated polypeptide chain of 165 amino acids and a molecular mass of 18409 Dalton. SCF is fused to a C-terminal His-tag (6x His) and purified by proprietary chromatographic techniques.
Species
Human
Product Overview
Human KITLG expressed in Sf9-insect cells
Molecular Mass
18.4 kDa
Size
CAT# CSC-CTK0292-10 (10 μg); CAT# CSC-CTK0292-50 (50 μg)
Expression System
Sf9-insect cells
Purity
Greater than 95% as determined by SDS-PAGE and HPLC analysis.
Activity
Recombinant SCF-Sf9 is fully biologically active when compared to a standard. The ED50 as determined by the dose-dependant stimulation of Human TF-1 cells is typically 2-5 ng/ml.
Endotoxin Level
Less than 1 EU/μg.
Formulation
Sterile-filtered, white lyophilized (freeze-dried) powder. Human SCF-Sf9 was lyophilized from a concentrated (1mg/ml) solution in 1X PBS (pH 7.4) with no additives.
Reconstitution
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu SCF-Sf9 should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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