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ActoFactor™ Recombinant Human Interleukin-8

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0053
  • Description
  • Interleukin-8 (IL-8) is a member of the a (CXC) subfamily of chemokines. It is produced by many cell types, including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, keratinocytes, hepatocytes, chondrocytes, and various tumor cell lines, in response to a wide variety of pro-inflammatory stimuli such as exposure to IL-1, TNF, LPS, and viruses. IL-8 is a potent chemoattractant for neutrophils, basophils, T cells and eosinophils. IL-8 also has pro-inflammatory effects, such as degranulation of neutrophil specific granules and azurophilic granules; induction of expression of the cell adhesion molecules CD11/CD18 and enhancement of adherence of neutrophils to endothelial cells and sub-endothelial matrix proteins. Beside neutrophils, IL-8 has been reported to be a co-mitogen for keratinocytes and was also shown to be an autocrine growth factor for melanoma cells. Recently, IL-8 was reported to be angiogenic both in vivo and in vitro. The 72 amino acid residue recombinant protein has a predicted molecular mass of approximately 8 kDa.
  • Species
  • Human
  • Product Overview
  • Human IL8 expressed in E.coli
  • Sequence
  • A DNA sequence encoding the mature human IL-8 protein was expressed in E. coli.
  • Size
  • CAT# CSC-CTK0053-10 (10 μg); CAT# CSC-CTK0053-50 (50 μg)
  • Expression System
  • E.coli
  • Purity
  • Greater than 97% as determined by SDS-PAGE and visualized by silver stain
  • Activity
  • Activity was determined by induction of myeloperoxidase release from human neutrophils, or chemotaxis of mouse BaF/3 cells transfected with human CXCR-2. The ED50 for these effects were typically 0.15 -0.3 μg/mL, or 0.1 - 0.5 ng/mL, respectively.
  • Endotoxin Level
  • Endotoxin level less than 0.1 ng per 1 μg of the cytokine as determined by the LAL method.
  • Formulation
  • Lyophilized from a sterile solution in PBS containing 50 μg of bovine serum albumin per 1 μg of cytokine.
  • Reconstitution
  • It is recommended that sterile PBS containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 10 μg/mL of the cytokine.
  • Storage & Stability
  • Lyophilized samples are stable for greater than six months at -20°C to -70°C. Reconstituted human IL-8/CXCL8 can be stored under sterile conditions at 2°C to 4°C for one month or at -20°C to -70°C for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.

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If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.

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    • USA
      45-1 Ramsey Road, Shirley, NY 11967, USA
      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
      Europe
      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com