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ActoFactor™ Recombinant Human Interleukin 6 receptor (HEK)

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0233
  • Description
  • IL-6 mediates its biological effects through the type I IL-6 receptor system that consists of two chains, IL-6R alpha and gp130. The IL-6R alpha chain is the binding component specific to IL-6; while the gp130 only transmits signals of IL-6 when bound to IL-6R alpha. The gp130 also can transmit signals from LIF, OSM, CNTF, IL-11 and CT-1 in conjunction with other receptor subunits. The low-affinity binding site for IL-6 is composed of IL-6R alpha alone. IL-6R alpha is expressed in a wide range of cells including T cells, fibroblasts and macrophages. Soluble IL-6R alpha which consists of only the extracellular domain of the IL-6R alpha chain, acts as an agonist of IL-6 activity at low concentrations. Recombinant human sIL-6R produced in E. coli is a 37.6 kDa protein consisting of the extracellular domain of the IL-6Rα chain (339 amino acid residues).
  • Species
  • Human
  • Product Overview
  • Human IL6R expressed in HEK293 cells
  • Molecular Mass
  • 37.6 kDa
  • Size
  • CAT# CSC-CTK0233-20 (20 μg); CAT# CSC-CTK0233-100 (100 μg)
  • Expression System
  • HEK293 cells
  • Purity
  • Greater than 98% as determined by SDS-PAGE and HPLC analysis.
  • Activity
  • Determined by its ability to intensify the IL-6 induced growth inhibition of mouse M1 cells. The expected ED50 is ≤ 5.0 ng/ml, in the presence of 20 ng/ml of rhIL-6.
  • Endotoxin Level
  • Less than 1 EU/μg.
  • Formulation
  • Sterile filtered through a 0.2 micron filter and lyophilized from 1x PBS (pH 7.2).
  • Reconstitution
  • Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile 1XPBS (pH 7.2) to a concentration of 0.1 -1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
  • Storage & Stability
  • The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

Citation Guidance:
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.

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      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
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      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com