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ActoFactor™ Recombinant Human Interleukin 5 (colony-stimulating factor, eosinophil)

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0055
  • Description
  • Human Interleukin 5 (IL-5) is a species-crossreactive cytokine produced by mast cells, T cells and eosinophils. It induces the proliferation, differentiation and activation of eosinophils. It is also a growth and differentiation factor for mouse but not human B cells. IL-5 is an antiparallel disulphide-linked homodimer of molecular mass of approximately 45 kDa. Mature IL-5 monomers contain 115 amino acid residues and 2 glycosylation sites. IL-5 binds to a specific heterodimeric cell surface receptor formed by two subunits, the binding IL-5Ra subunit and the non-binding b subunit, also shared by the receptors for IL-3 and GM-CSF, thus termed b-common (bc). Due to heterogeneous glycosylation, recombinant IL-5 resolves into multiple bands ranging from 32-34 kDa in SDS-PAGE.
  • Species
  • Human
  • Product Overview
  • Human IL5 expressed in Sf21 insect cells
  • Sequence
  • A DNA sequence encoding the human IL-5 protein sequence was expressed in Sf 21 insect cells.
  • Size
  • CAT# CSC-CTK0055-5 (5 μg); CAT# CSC-CTK0055-25 (25 μg)
  • Expression System
  • Sf21 insect cells
  • Purity
  • Greater than 97% as determined by SDS-PAGE and visualized by silver stain
  • Activity
  • Activity was determined in a cell proliferation assay using a factor-dependent human cell line, TF-1 and the ED50 for this effect was typically 0.1 - 0.2 ng/mL.
  • Endotoxin Level
  • Endotoxin level less than 0.1 ng per 1 mg of the cytokine as determined by the LAL method.
  • Formulation
  • Lyophilized from a 0.2 μm filtered solution in PBS containing 50 mg of bovine serum albumin per 1 mg of cytokine.
  • Reconstitution
  • It is recommended that sterile phosphate-buffered saline containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 1 mg/mL of the cytokine.
  • Storage & Stability
  • Lyophilized samples are stable for greater than six months at -20°C to -70°C. Reconstituted IL-5 can be stored under sterile conditions at 2°C - 4°C for one month or at -20°C to -70°C for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.

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      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
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      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com