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ActoFactor™ Recombinant Human Interleukin 5

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0228
  • Description
  • Interleukin-5 (IL-5), also known as eosinophil differentiation factor (EDF), is a lineage-specific cytokine for eosinophilpoiesis. It regulates eosinophil growth and activation, and thus plays an important role in diseases associated with increased levels of eosinophils, including asthma. IL-5 has a similar overall fold to other cytokines (e.g. IL-2, IL-4 and GCSF), but while these exist as monomeric structures, IL-5 is a homodimer. Recombinant human IL-5 produced in E. coli is a dimeric, non-glycosylated polypeptide containing two 113 amino acids chains with a molecular mass of 26,523 Dalton.
  • Species
  • Human
  • Product Overview
  • Human IL5 expressed in E.coli
  • Molecular Mass
  • 26.5 kDa
  • Size
  • CAT# CSC-CTK0228-10 (10 μg); CAT# CSC-CTK0228-50 (50 μg)
  • Expression System
  • E.coli
  • Purity
  • Greater than 98% as determined by SDS-PAGE and RP-HPLC analysis.
  • Activity
  • Recombinant human IL-5 is fully biologically active when compared to a standard. The ED50, as determined by the dose-dependant stimulation of the proliferation of TF-1 cells, was found to be less then 0.15 ng/ml corresponding to a specific activity of 6 x 10^6 IU/mg.
  • Endotoxin Level
  • Less than 1 EU/μg.
  • Formulation
  • Sterile-filtered, white, lyophilized (freeze-dried)powder. The protein was lyophilized from a concentrated (1 mg/ml), sterile solution with no additives.
  • Reconstitution
  • Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1 - 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
  • Storage & Stability
  • The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu IL-5 should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

Citation Guidance:
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.

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    • USA
      45-1 Ramsey Road, Shirley, NY 11967, USA
      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
      Europe
      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com