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ActoFactor™ Recombinant Human Interleukin 2 receptor, alpha (Insect)

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0304
  • Description
  • The IL-2 receptor system consists of three non-covalently linked subunits termed IL-2R alpha, IL- 2R beta, and IL-2R gamma. The IL-2R alpha is a type I transmembrane protein consisting of a 219 amino acid (a.a.) extracellular domain, a 19 a.a. transmembrane domain and a 13 a.a. intracellular domain, which is not involved in the transduction of IL-2 signals. Proteolytic processing of IL-2R alpha releases the entire extracellular domain of IL-2R alpha thereby generating a 219 a.a. soluble protein called soluble IL-2R alpha (sIL-2R alpha). The homodimeric form binds IL-2 (KD=10 mM) and facilitates IL-2 signaling. The secreted sIL-2R alpha is expressed on leukemia cells, lymphoma cells, newly activated T and B cells, as well as on approximately 10% of NK cells. Recombinant human sIL-2R alpha is a 24.8 kDa protein containing 219 amino acid residues consisting of only the extracellular domain of IL-2R-alpha. Due to glycosylation, IL-2R-alpha has an approximate molecular weight of 31 kDa based on SDS-PAGE gel and mass spectrometry.
  • Species
  • Human
  • Product Overview
  • Human IL2RA expressed in (BTI-Tn-5B1-4) Hi-5 insect cells
  • Molecular Mass
  • 24.8 kDa
  • Size
  • CAT# CSC-CTK0304-25 (25 μg); CAT# CSC-CTK0304-125 (125 μg)
  • Expression System
  • (BTI-Tn-5B1-4) Hi-5 insect cells
  • Purity
  • Greater than 98% as determined by SDS-PAGE and HPLC analyses.
  • Activity
  • Determined by its ability to increase the proliferation effect of IL-2 in mouse CTLL-2 cells. In the presence of 1 ng/ml of recombinant IL-2, the expected ED50 for this effect is between 0.5-1.5 μg/ml.
  • Endotoxin Level
  • Less than 1 EU/μg.
  • Formulation
  • Sterile filtered through a 0.2 micron filter and lyophilized from 1x PBS.
  • Reconstitution
  • Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile 1x PBS (pH 7.2) to a concentration of 0.1 - 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
  • Storage & Stability
  • The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored dessicated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at –20°C. For long term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze / thaw cycles.

Citation Guidance:
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.

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      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
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      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com