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ActoFactor™ Recombinant Human Fibroblast growth factor 2 (basic) (E. coli)

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0134
  • Description
  • Recombinant Human basic Fibroblast Growth Factor (rHu bFGF) is a heparin binding growth factor, which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. rHu bFGF also exerts a potent angiogenic activity in vivo. Human bFGF is a 16.5 kDa protein containing 153 amino acid residues.
  • Species
  • Human
  • Product Overview
  • Human FGF2 expressed in E.coli
  • Molecular Mass
  • 16.5 kDa
  • Size
  • CAT# CSC-CTK0134-50 (50 μg); CAT# CSC-CTK0134-250 (250 μg)
  • Expression System
  • E.coli
  • Purity
  • Greater than 98% as determined by SDS-PAGE analysis.
  • Activity
  • The ED50, as determined by the stimulation of cell proliferation of human umbilical vein endothelial cells (HUVEC) by FGF-2, is in the range of 0.1-2.0 ng/ml, corresponding to a specific activity of 5 x 10? to 1.0 x 10^7 units/mg (IU/mg). The measured biological activity was identical with reference standards which showed an ED50 ≤0.1 ng/ml (corresponding to a specific activity of Greater than 1.0 x 10^7 units/mg and determined by the stimulation of 3H-thymidine incorporation in BALB/c 3T3 cells).
  • Endotoxin Level
  • N/A
  • Formulation
  • Lyophilized from a sterile-filtered protein solution in PBS with no additives.
  • Reconstitution
  • Please centrifuge product briefly before opening the vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1 - 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
  • Storage & Stability
  • The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu bFGF should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long term storage, carrier protein should be added to avoid loss of bioactivity (0.1% HSA or BSA). Please avoid repeated freeze-thaw cycles.

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      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
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      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com