- Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The alpha-defensins are distinguished from the beta-defensins by the pairing of their three disulfide bonds. To date, four human beta-defensins have been identified; BD-1, BD-2, BD-3 and BD-4. Beta-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they are chemoattractant towards immature dendritic cells and memory T cells. The Beta-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence and, in the case of BD-1 (36 a.a.), a propeptide region. Beta-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Beta-Defensins are 3-5 kDa peptides ranging in size from 33-47 amino acid residues. Recombinant human BD-3 is a 5.1 kDa protein containing 45 amino acid residues.
- Product Overview
- Human DEFB103B expressed in E.coli
- CAT# CSC-CTK0435-20 (20 μg); CAT# CSC-CTK0435-100 (100 μg)
- Greater than 95% as determined by SDS-PAGE, FPLC and HPLC analysis.
- Exhibits antimicrobial activity against gram-positive bacteria S. aureus and gram-negative P. aeruginosa and E. coli.
- Endotoxin Level
- Less than 1 EU/μg.
- Lyophilized from a sterile-filtered solution with no additives.
- Please centrifuge product briefly before opening the vial. Reconstitute in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can be diluted into other aqueous buffers and stored at -20°C for future use.
- Storage & Stability
- The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted BD-3 should be used immediately or stored in undiluted working aliquots at -20°C. Avoid repeated freeze / thaw cycles.
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