- Among CXC chemokines, CXCL10 has been identified to play an important role in several endocrinological autoimmune diseases such as Hashimotos thyroiditis, Graves disease and Type 1 diabetes mellitus. The chemokine IP-10 (interferon-inducible protein of 10 kDa, CXCL10) is a chemoattractant for CXCR3+ T cells, binds to the G protein-coupled receptor CXCR3, which is found mainly on activated T cells and NK cells, and plays an important role in Th1-type inflammatory diseases. IP-10 also binds to glycosaminoglycans (GAGs), an interaction thought to be important for its sequestration on endothelial and other cells. Recombinant human IP-10 produced in E. coli is a single, non-glycosylated polypeptide chain of 77 amino acids and a molecular mass of 8,516 Dalton.
- Product Overview
- Human CXCL10 expressed in E.coli
- CAT# CSC-CTK0447-25 (25 μg); CAT# CSC-CTK0447-125 (125 μg)
- Greater than 97% as determined by SDS-PAGE and RP-HPLC analysis.
- Determined by its ability to chemoattract human T-Lymphocytes using a concentration range of 10-100 ng/ml.
- Endotoxin Level
- Less than 1 EU/μg.
- Lyophilized from a sterile-filtered solution in 20 mM PBS (pH 7.4).
- Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
- Storage & Stability
- The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu IP-10 should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.