Online Inquiry & Support

USA: 1-631-626-9181   Fax: 1-631-614-7828
Europe: 44-207-097-1828
Email: info@creative-bioarray.com

ActoFactor™ Recombinant Human Chemokine (C-C motif) ligand 2

Bookmark and Share
  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0038
  • Description
  • Monocyte chemotactic protein-1 (MCP-1) is a member of the b (CC) family of chemokines. The protein is identical to the product of the human JE gene which is a platelet-derived growth factor (PDGF)-inducible gene. MCP-1 is produced by fibroblasts, tumor cells, smooth muscle cells, endothelial cells, and mononuclear phagocytes. MCP-1 is a chemoattractant for monocytes. In addition, it activates monocytes to be cytostatic for some human tumor cells lines; it increases cytosolic free calcium; it generates and releases monocyte superoxide anions and monocyte lysosomal enzymes in vitro. MCP-1 was reported to be capable of regulating adhesion molecule expression and cytokine production in human monocytes as well as chemoattracting, activating, and inducing histamine release from basophils. MCP-1 can bind to CCR1 and a specific receptor cloned from THP-1 and MonoMac 6 cells. Human MCP-1 cDNA encodes a 99 amino acid residue precursor protein with a 23 residue hydrophobic signal peptide that is cleaved to generate the 76 residue mature protein. The recombinant mature protein has a predicted molecular mass of approximately 8.7 kDa.
  • Species
  • Human
  • Product Overview
  • Human CCL2 expressed in E.coli
  • Sequence
  • A DNA sequence encoding mature human MCP-1 was expressed in E. coli.
  • Size
  • CAT# CSC-CTK0038-10 (10 μg); CAT# CSC-CTK0038-50 (50 μg)
  • Expression System
  • E.coli
  • Purity
  • Greater than 97% as determined by SDS-PAGE and visualized by silver stain
  • Activity
  • Activity was determined by monocyte chemotaxis and the ability to chemoattract mouse BaF/3 cells transfected with human CCR2A. The ED50 for these effects were typically 5 - 20 ng/mL and 10 - 60 ng/mL, respectively.
  • Endotoxin Level
  • Endotoxin level less than 0.1 ng per 1 mg of the cytokine as determined by the LAL method.
  • Formulation
  • Lyophilized from a sterile solution in PBS containing 50 mg of bovine serum albumin per 1 mg of cytokine.
  • Reconstitution
  • It is recommended that sterile PBS containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 1 mg/mL of the cytokine.
  • Storage & Stability
  • Lyophilized samples are stable for greater than six months at -20°C to -70°C. Reconstituted human MCP-1/CCL2 can be stored under sterile conditions at 2°C to 4°C for one month or at -20°C to -70°C for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Citation Guidance:
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.

Price Inquiry

Please input "bioarray" as verification code.

SEARCH

tissue samples, tissue arrays, cells, microorganisms, probes and services for your research.

  

SEARCH

Contact Us
    • USA
      45-1 Ramsey Road, Shirley, NY 11967, USA
      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
      Europe
      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
Ordering Information
    • Payment methods we support:
      ✭ Invoice / Purchase Order
      ✭ Credit card
Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com