- CD14 (also known lipopolysaccharide [LPS] receptor) is expressed strongly on monocytes and macrophages and weakly on the surface of neutrophils. CD14 (1-201 a.a.) is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for complexes of LPS and LPS binding protein (LBP). Soluble CD14, also binding to LPS, acts at physiological concentrations as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell activation. The myeloid differentiation antigen CD14 acts as the major receptor for bacterial LPS. The dominant form of the recombinant wildtype CD14 is the 50 kDa protein.|The rHuCD14 is produced from human CD14-transfected CHO-cells. Before transfection, the complete human CD14-cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR.
- Product Overview
- Human CD14 expressed in CHO cells
- CAT# CSC-CTK0300-50 (50 μg); CAT# CSC-CTK0300-250 (250 μg)
- Expression System
- CHO cells
- Greater than 95% as determined by SDS-PAGE analysis.
- Up to 20 μg/ml CD14 inhibit binding of FITC-LPS (0.5 μg/ml) to 6 x 10^5 CD14+CHO transfectants (FACS).
- Recombinant human CD-14 was lyophilized from a concentrated protein solution (1 mg/ml) containing phosphate-buffered saline, pH 7.2.
- Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0,1 - 1,0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
- Storage & Stability
- The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored dessicated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at –20°C. For long term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze / thaw cycles... Up to 20 μg/ml CD14 inhibit binding of FITC-LPS (0.5 μg/ml) to 6 x 10? CD14+CHO transfectants (FACS).
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