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ActoFactor™ Recombinant Human Bone morphogenetic protein 4 (E. coli)

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0529
  • Description
  • Human Bone Morphogenetic Protein-2 (BMP-2) is a disulfide-bonded homo-dimeric protein with an apparent molecular weight of 26 kDa. BMP-4 regulates similarly to its nearest homologue BMP-2, diverse fundamental processes during embryonic development. BMP-4 and other BMP proteins are members of the transforming growth factor-beta (TGF-beta) superfamily of proteins and have great potential for medical therapeutic applications, in particular because they allow or at least accelerate the ossification of extensive bone lesions. Recombinant human BMP-4 produced in E. coli is a monomeric, non-glycosylated polypeptide chain of 116 amino acids and a molecular mass of 13,009 Dalton.
  • Species
  • Human
  • Product Overview
  • Human BMP4 expressed in E.coli
  • Molecular Mass
  • 13 kDa
  • Size
  • CAT# CSC-CTK0529-10 (10 μg); CAT# CSC-CTK0529-50 (50 μg)
  • Expression System
  • E.coli
  • Purity
  • Greater than 95% as determined by SDS-PAGE and HPLC analysis.
  • Activity
  • N/A
  • Endotoxin Level
  • Less than 1 EU/μg.
  • Formulation
  • Sterile-filtered, white, lyophilized (freeze-dried) powder. Lyophilized from a sterile-filtered, concentrated protein solution (1 mg/ml) in 30% acetonitrile and 0.1% TFA.
  • Reconstitution
  • Please centrifuge product briefly before opening vial. The lyophilized protein can be reconstituted in sterile, ultra-pure water to a concentration of 0.1 - 1.0 mg/ml (for highly concentrated dilutions we recommend reconstitution in sterile 100 mM acetic acid). This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
  • Storage & Stability
  • The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu BMP-4 should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles. Applications Can be used as a molecular standard (Western, ELISA) for studying secreted BMP-4, for preparing antibodies for the BMP-4 monomer and as molecule standard for the detection of secreted BMP-4 in reduced SDS-PAGE.

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      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
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      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com