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ActoFactor™ Recombinant Human Bone morphogenetic protein 4

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0039
  • Description
  • BMP-4 (Bone Morphogenetic Protein 4) is a member of the transforming growth factor β (TGF-β) superfamily. BMPs have been shown to be key regulators of embryogenesis and are known to play a role in the growth and differentiation of various cell types, including embryonic stem cells (ESCs), mesenchymal cells, epithelial cells, hematopoietic cells, and neuronal cells. The mature recombinant human BMP-4 is a disulfide-linked homodimeric protein consisting of two 116 amino acid residue subunits, and is generated by the proteolytic removal of the signal peptide and propeptides. The monomer has a calculated molecular mass of approximately 13 kDa, but migrates as an approximately 22 kDa protein under reducing conditions in SDS-PAGE due to glycosylation.
  • Species
  • Human
  • Product Overview
  • Human BMP4 expressed in NS0 cells
  • Sequence
  • The DNA sequence encoding the human BMP-2 signal peptide and propeptide (amino acid residues 1 - 282 of human BMP-2) fused to the human BMP-4 mature chain (amino acid residues 293 - 408 of human BMP-4) (Wozney, J. et al., 1988, Science 242: 1528 - 1534) w
  • Size
  • CAT# CSC-CTK0039-10 (10 μg); CAT# CSC-CTK0039-50 (50 μg)
  • Expression System
  • NS0 cells
  • Purity
  • Greater than 95% as determined by SDS-PAGE and visualized by silver stain
  • Activity
  • The ED50 as measured by its ability to induce alkaline phosphatase production by mouse ATDC5 chondrogenic cells (Nakamura, K. et al., 1999, Exp. Cell Res. 250: 351) is typically 10 - 30 ng/mL.
  • Endotoxin Level
  • Endotoxin level is less than 1.0 EU per 1 μg of the cytokine, as determined by the LAL method.
  • Formulation
  • Lyophilized from a sterile-filtered solution in 30% acetonitrile, 0.1% TFA containing 50 μg of bovine serum albumin per 1 μg of cytokine.
  • Reconstitution
  • It is recommended that sterile 4 mM HCl containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 10 μg/mL.
  • Storage & Stability
  • The lyophilized powder is stable for greater than six months at -20°C to -70°C. Reconstituted BMP-4 can be stored under sterile conditions at 2°C to 4°C for one month or at -20°C to -70°C for three months without detectable loss of activity. Avoid repeated freezing and thawing.

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    • USA
      45-1 Ramsey Road, Shirley, NY 11967, USA
      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
      Europe
      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com