- Human Bone Morphogenetic Protein-2 (BMP-2) is a member of the TGF-beta superfamily of cytokines that effect bone and cartilage formation. Human BMP-2 is a disulphide linked homodimeric protein with an apparent molecular weight of 26 kDa. BMP-2 regulates similarly to its nearest homologue BMP-4, diverse fundamental processes during embryonic development, including induction of bone and cartilage growth and regeneration, cardiac cell differentiation and epithelial to mesenchymal transition. BMP-2 promotes osteogenic and chondrogenic differentiation from mesenchymal cells, stem cells, and vascular smooth muscle cells. BMP-2 and other BMP proteins have great potential for medical therapeutic applications, in particular because they allow or at least accelerate the ossification of extensive bone lesions.
- Product Overview
- Human BMP2 expressed in Nicotiana tobacum
- CAT# CSC-CTK0525-10 (10 μg); CAT# CSC-CTK0525-50 (50 μg)
- Expression System
- Nicotiana tobacum
- Each is tested for bioactivity and verified to have comparable activity to a commercial source. The bioactivity of recombinant human BMP-2 was determined by its dose-dependent induction of alkaline phosphatase production by ATDC-5 mouse chondrogenic cells. The ED50 value for this batch of BMP-2 was determined to be below 75 ng/ml, corresponding to a specific activity of >13,300 units/mg. Optimal concentration should be determined for specific applications and cell lines.
- Endotoxin Level
- Less than 0.05 EU/μg.
- Lyophilized from a concentrated, sterile protein solution containing 25 mM MES (pH 6.0).
- Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultrapure water to a concentration no less than 50 μg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use. Due to the protein nature, dimmers and multimers may be observed. Optimal concentration should be determined for specific application and cell lines.
- Storage & Stability
- The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu BMP-2 should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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