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SF-21

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-C6196X
  • Description
  • Derived from pupal ovarian tissue of spodoptera frugiperda. The cells are highly susceptible to Baculovirus infection and are used in the production of protein products genetically manipulated into Baculovirus vector systems. They were derived at the USDA Insect Pathology Laboratory from a cell line originating from pupal ovarian tissue of the fall army worm, Spodoptera frugiperda. Sf21 cells have greater range in size and have a doubling time of 24 hr compared to 48-72 hr for Sf9 cells
  • Recommended Medium
  • 80-90% TC 100 medium + 10-20% h.i. FBS (or 80-90% Grace′s insect medium + 10-20% h.i. FBS supplemented with L-Glutamine, hydrolyzed lactalbumin and yeastolate)
  • Organism
  • Spodoptera frugiperda, Fall armyworm
  • Morphology
  • Spherical
  • Culture Properties
  • suspension
  • Source
  • Ovary
  • Quality Control
  • Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
    Viruses: PCR: SMRV -
  • Storage and Shipping
  • Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
  • Application
  • This cell line can be used for protein expression using the Baculovirus vector system

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    • USA
      45-1 Ramsey Road, Shirley, NY 11967, USA
      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
      Europe
      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com