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MFM-223

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  • Cat.No.
  • CSC-C0461
  • Description
  • Established from the pleural effusion of a post-menopausal breast cancer patient without any prior treatment (tumor: widespread ductal mammary carcinoma T4 N2 M1 with histological grade III); cells were described to express androgen, estrogen and progesterone receptors
  • Recommended Medium
  • 85% MEM (with Earles salts) + 15% h.i. FBS + 2 mM L-glutamine + 1x insulin-transferrin-sodium selenite
  • Organism
  • Human
  • Morphology
  • adherent cells growing as monolayer with reticulate growth pattern
  • Culture Properties
  • monolayer
  • Karyotype
  • Human highly rearranged near-diploid karyotype with 12% polyploidy - 46(45-46)<2n>X, -X, -2, -5, -6, +7, -9, -10, +11, +12, -14, -15, -16, -17, -18, -19, -22, +10mar, add(5)(p15), der(7)?inv(7)(q1?q2?)dup(7)(q31q35), add(7)(q11;hsr), der(7)add(7)(p22)add(
  • Quality Control
  • Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
    Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -,
  • Storage and Shipping
  • Frozen with 70% medium, 20% FBS, 10% DMSO at about 0.8 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
  • Tissue
  • breast carcinoma

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Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-048-3343
Email: info@creative-bioarray.com