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KB-V1

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-C6212X
  • Description
  • KB-V1 is a multidrug-resistant (mdr) subclone derived in 1985 from KB-3-1 which itself is a derivative of the 1951 established HELA (31-year-old black woman); cells express strongly mdr1 mRNA and protein; the cell line will only be distributed to non-profit institutions, commercial entities may apply for a license at the NIH/NCI Technology Transfer Cernter, Rockville, MD, USA, phone (301)435-3111
  • Recommended Medium
  • 85% DMEM + 15% h.i.FBS + 200-1000 ng/ml vinblastine
  • Organism
  • Human
  • Morphology
  • epitheloid-like, sometimes round cells growing in loosely adherent monolayers when vinblastine is present
  • Culture Properties
  • monolayer
  • Karyotype
  • Human flat-moded hypotriploid karyotype with 13% polyploidy - 71-77<3n>XX, -X, +1, +3, +5, +5, +5, +7, +7, +8, +8, -9, -11, +12, +15, -16, +17, -18, -19, +21, -22, +mar, der(1;3)(q10;p10), del(3)(q13), der(3;5)(p10;q10), i(5p)x3, del(7)(q10), der(7)add(7)
  • Source
  • cervix carcinoma (derivative of HELA)
  • Quality Control
  • Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
    Immunology: cytokeratin +, desmin -, endothel -, GFAP -, neurofilament -, vimentin +
    Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, H
  • Storage and Shipping
  • Frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

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      45-1 Ramsey Road, Shirley, NY 11967, USA
      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
      Europe
      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com