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HPB-ALL

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Cat.No.
CSC-C0497
Description
Established from the peripheral blood of a 14-year-old Japanese boy with ALL and thymoma at diagnosis in 1973
Species
Human
Source
T cell leukemia
Recommended Medium
80-90% RPMI-1640 + 10-20% h.i. FBS
Culture Properties
suspension
Morphology
round to polymorph cells growing singly or in clusters in suspension
Karyotype
Pseudodiploid human karyotype with 8% polyploidy - 46<2n>XY, der(1)t(1;16)(q22;p11-12)add(16)(p13), del(2)(p24), del(3)(p11), der(5)t(1;5)(q22-24;q32-33), r(16)(?p12?q12) - sideline with -3 instead of del(3) - resembles published karyotype
Quality Control
Mycoplasma: negative in microbiological culture, RNA hybridization, PCR assays
Immunology: CD2 +, CD3 +, CD4 +, CD5 +, CD6 +, CD7 +, CD8 +, CD13 -, CD19 -, CD34 -, TCRalpha/beta +, TCRgamma/delta -
Viruses: PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/II -, S
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.

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Q:
What is an ISH assay?
A:

In situ hybridization (ISH) is a technique that uses colorimetric or fluorescent probes to target and visualize specific DNA or RNA sequences within tissues. This technology can be broadly applied to study infectious agents, cancer, or developmental biology.

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Average Rating: 5.0    |    1 Scientist has reviewed this product

Good results

We culture the cells in vitro, which can be used as a reliable model for studying lymphoma and its progression.

11 Apr 2023


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