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  • Cat.No.
  • CSC-C0345
  • Description
  • Established from the biopsy specimens from a 42-year-old woman with glioma (histologically defined as glioblastoma)
  • Recommended Medium
  • 90% DMEM + 10% h.i. FBS + 2 mM L-glutamine + 4x MEM non-essential amino acids
  • Organism
  • Human
  • Morphology
  • adherent, large, spindle-like cells growing in monolayers
  • Culture Properties
  • monolayer
  • Karyotype
  • Human hypotriploid karyotype with 17% polyploidy - 66(61-67)<3n>XXX, -4, +5, +7, -10, -13, -19, -22, +dm x1-2, der(1)t(1;4)(q11;p11), del(3)(p14), der(10)t(3;10)(p14;q13), del(17)(p11), del(18)(p11) - additional subclonal rearrangements, add(2)(q11), add(
  • Quality Control
  • Mycoplasma: contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
    Immunology: cytokeratin -, cytokeratin-7 -, cytokeratin-8 -, cytokeratin-17 -, cytokeratin-18 -, desmin -,
  • Storage and Shipping
  • Frozen with 70% medium, 20% FBS, 10% DMSO at about 2.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
  • Tissue
  • glioma

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USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-048-3343
Email: info@creative-bioarray.com