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DLD-1

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-C9371L
  • Description
  • DLD-1 is one of two colorectal adenocarcinoma cell lines which were isolated by D.L. Dexter and associates during a period from 1977-1979. The cells have been used in the study of polar solvents on cell characteristics. The four cell lines: DLD-1, HCT-15, HCT-8 and HRT-18 have been shown to have a common genetic origin. DLD-1 can express carcinoembryonic antigen (CEA) 0.5 ng/106 cells/10 days and colon antigen 3. These cells are weakly positive for keratins and vimentin. The cells are positive for keratin by immunoperoxidase staining.
  • Organism
  • Homo sapiens, human
  • Morphology
  • Epithelial
  • STR DNA Profile
  • Amelogenin: X,Y
    CSF1PO: 11,12
    D13S317: 8,11
    D16S539: 12,13
    D5S818: 13
    D7S820: 10,12
    THO1: 7,9.3
    TPOX: 8,11
    vWA: 18,19
  • Culture Properties
  • monolayer
  • Karyotype
  • This is a pseudodiploid human cell line with the modal chromosome number of 46, occurring in 86% of cells. The rate of polyploidy was high at 17.1%. The karyotype of the line was 46,XY,-2,+dir dup(2)(p13-p23). The Y chromosome was slightly longer than N22 and had a large segment of heterochromatic, fluorescent distal q arms.
  • Source
  • Colon
  • Quality Control
  • Tests for mycoplasma, bacteria and fungi were negative
  • Storage and Shipping
  • Frozen with 52.5% RPMI-1640, 40% FBS, 7.5% DMSO at about 4-5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
  • Application
  • This cell line can be used as a suitable transfection host.
  • Disease
  • Colon adenocarcinoma

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      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
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      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com