COLO-678

Specification
Recommended Products

Cat.No.

CSC-L0003

Description

Established from a metastatic lymph node of a 69-year-old man with colon carcinoma in 1984

Species

Human

Source

colon carcinoma

Recommended Medium

90% RPMI-1640 + 10% h.i. FBS

Culture Properties

monolayer

Morphology

round or spindle-shaped adherent cells growing as monolayer

Karyotype

Human hyperdiploid karyotype with 4% polyploidy - 57(52-58)<2n>XXY, +X, +3, +7, +8, +10, +11, +12, +15, -17, -18, +22, +4mar, add(3)(p11), amp(7)(q11.23)x2, del(12)(q21/22q24.2)x2 - trisomy 7 and 12 are associated with colon carcinoma

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -,

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

CM-1448Y

SuperCult® RPMI 1640 Medium

INQUIRY

CS-001Y

PremSera® FBS

INQUIRY

For research use only. Not for any other purpose.