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COLO 320 HSR

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-C9363L
  • Description
  • CEA(-), CSAp(-), colon antigen 3(+); This line is a subline of COLO 320DM. The cells are weakly positive for keratins and vimentinTumorigenecity:yes, in nude miceIsoenzyme:PGM1, 1; PGM3, 2; G6PD, B; PEP-D, 1; 6PGD, A;ES-D, 1Histopathology:adenocarcinoma; DukesSubculture:Shake flask, pour one-half of the medium into a new flask and add fresh medium to both flasks. Cells remaining attached may be removed (if desired) using a standard trypsin protocol. Subculture once per week.Split ratio:Media change:Reverse transcritase:negativeProductionserotonin; norepinephrine; epinephrine; adrenocorticotropic hormone(ACTH); parathyroid hormone
  • Organism
  • Human
  • Morphology
  • rounded and refractile
  • STR DNA Profile
  • Amelogenin: X
    CSF1PO: 11
    D13S317: 11
    D16S539: 11/12
    D5S818: 12
    D7S820: 9/12
    THO1: 9
    TPOX: 8/9
    vWA: 15/18
    D3S1358: 17
    D21S11: 33.2
    D18S51: 15
    Penta E: 11
    Penta D: 9/12
    D8S1179: 13
    FGA: 20 , 2010.
  • Culture Properties
  • The cells grows loosely attached and in suspension
  • Source
  • colon
  • Quality Control
  • Tests for mycoplasma, bacteria and fungi were negative
  • Storage and Shipping
  • Frozen with 52.5% RPMI-1640, 40% FBS, 7.5% DMSO at about 4-5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com