Establishment Protocol for Mouse Model of Granulomonocytic Leukemia (WEHI-3)
GUIDELINE
The granulomonocytic leukemia (WEHI-3) mouse model is an acute non-lymphocytic leukemia model constructed from mice. The basic principle of this model is that mineral oil is injected into mice to create an in vivo environment that produces leukemia. It can be used for the study of human monocytic leukemia and granulomonocytic leukemia, as well as for the study of splenic colonization in vivo.
METHODS
- Eighteen 7-week-old male BALB/c mice were injected intraperitoneally with 0.4 mL of medical liquid paraffin, and the injection was repeated once at 11 weeks of age and once at 15 weeks of age; testosterone propionate 0.01 mg (dissolved in 0.05 ml of olive oil) was injected subcutaneously between 7 weeks of age and 14 weeks of age five times per week, and then testosterone 0.25 mg was injected once at 17 weeks of age, and tumors developed in 11 of these mice, referred to as WEHI-3.
- Single-cell suspensions were prepared from solid tumors taken from primary granulomonocytic leukemia mice and transplanted to 8 recipient mice, all of which developed tumors.
- 17~21 days after transplantation, 4 splenocyte cell suspensions were taken and transplanted again, and each suspension was injected into 4 recipient mice, and the transplantation was continued for 20 days for the first 3 transplants, and 14 days for the subsequent transplants, resulting in 4 sublines of WEHI-3A, B, C, and D. The four sublines, WEHI-3A, B, C, and D, were obtained by transplantation.
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NOTES
- Follow institutional guidelines and obtain necessary approvals for animal experimentation.
- Regularly monitor the injected mice for signs of disease progression, including weight loss, lethargy, and other clinical symptoms characteristic of granulomonocytic leukemia. Collect blood samples for hematological analysis and monitor cell counts, differential counts, and leukemia cell infiltration.
- Ensure proper documentation and record-keeping throughout the experimental procedures, including detailed information on procedures, observations, and results.