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Europe: 44-207-048-3343
Email: info@creative-bioarray.com

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Custom iPS Cell Services

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Many of our customers use our source to develop iPSC lines from our primary cells and to develop assays as well as to do full disease and developmental studies.

Multiple reprogramming methods

A broad range of reprogramming systems is available including mRNA reprogramming, dox-inducible human 4F2A reprogramming and lentivirus reprogramming. These technologies have been adopted and developed in close collaboration with leading iPS cell pioneers. Our extensive range of products allows for customization of individual transcription factors, cell culture conditions, and delivery systems for the optimization of reprogramming parameters.

How the iPS Cell Lines are made

Service Details

A.Retrovirus based iPSC reprogramming protocol

  1. Retroviral reprogramming using standard Oct4, Sox2, c-Myc, and Klf4 Factors
  2. iPSC colonies are selected based on morphology
  3. AP Staining and immunostaining of ESC markers (Nanog or Oct4, SSEA3 and TRA-1-81 included)
  4. 5 vials iPSC colonies will be provided (>2 x 10^5 cells/vial)
  5. Timelines for human iPSCs: 3-4 months and mouse iPSCs: 2-3 months

B. Episomal vector based iPSC reprogramming protocol

  1. Episomal DNA reprogramming using Oct4, Sox2, c-Myc, and Klf4 factors with small molecules
  2. iPSC colonies are selected based on morphology
  3. AP Staining and immunostaining of ESC markers (Nanog or Oct4, SSEA3 and TRA-1-81 included)
  4. 5 vials iPSC colonies will be provided (>2 x 10^5 cells/vial)
  5. Timelines for human iPSCs: 4-5 months

C. Protein based iPSC reprogramming protocol

  1. Protein reprogramming using standard Oct4, Sox2, c-Myc, and Klf4 factors with a cell-penetrating peptide (CPP)
  2. iPSC colonies are selected based on morphology
  3. AP Staining and immunostaining of ESC markers (Nanog or Oct4, SSEA3 and TRA-1-81 included)
  4. iPSC colonies will be provided (>5 x 10^5 cells/vial)
  5. Timelines for human iPSCs: 3-4 months

Creative Bioarray's Custom Reprogramming Services empower you to focus on your experiments and not with consuming lab time and resources in generating iPS cells prior to beginning experiments. If you have any special needs in generating iPS cells by multiple reprogramming methods, please contact us at info@creative-bioarray.com or 1-631-626-9181 for this special service. Let us know what you need and we will accommodate you. We look forward to working with you in the future.

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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-048-3343
Email: info@creative-bioarray.com