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WiDr

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Cat.No.
CSC-C0258
Description
According to Chen TR, 1987, the WiDr cell line is a derivative of the HT-29 cell line. HT29 was isolated from a primary colon adenocarcinoma in a 44-year-old Caucasian female. The cells are negative for Colon Antigen 3 expression, but positive for keratin by immunoperoxidase staining. The cells expressed p53 antigen (the p53 produced has a G-A mutation resulting in Arg-His at position 273). Growth of WiDr cells is inhibited by tumor necrosis factor alpha (TNF Inhibitors of dihydrofolate reductase are highly cytotoxic to WiDr cells). WiDr were also described to express epidermal growth factor (EGF).
Organism
Homo sapiens, human
STR DNA Profile
Amelogenin: X,X
CSF1PO: 11,12
D13S317: 11,11
D16S539: 11,12
D5S818: 11,12
D7S820: 10
THO1: 6,6
TPOX: 8,9
vWA: 17,19
D3S1358: 15,17
D21S11: 29,30
D18S51: 13
Penta E: 14,16
Penta D: 11,13
D8S1179: 10
FGA: 20,22
Source
Colon
Culture Properties
monolayer, adherent
Morphology
Epithelial
Recommended Medium
SuperCult® WiDr Medium
Quality Control
Tests for mycoplasma, bacteria and fungi were negative
Storage and Shipping
liquid nitrogen vapor phase
Disease
Colorectal adenocarcinoma
Application
WiDr can produce carcinoembryonic antigen and are tumorigenic in xenogeneic animals. These cells have been used in studies of anti-tumour agents.

Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
CSC-6317W
CSC-C0204
CSC-C0253
CSC-C0256
CSC-C0300
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