Derived from the pleural effusion of a 41-year-old Caucasian man with B-cell non-Hodgkin lymphoma (B-NHL, diffuse large cell lymphoma non-cleaved type, intermediate grade - transformed from follicular small cleaved cell, low grade) at the status of relapse/progressive disease in 1990; cell line carries EZH2 Y641F mutation
Human hyperdiploid karyotype - 47(43-48)<2n>XY, +7, +8, t(1;2)(p36;q37), del(3)(q25), t(4;14)(q27;q31), der(7)t(3;7)(q25;p13), i(7p), add(8)(q24)x2, del(13)(?q21?q22), t(14;18)(q32;q21), del(15)(q25q27) - carries t(14;18) effecting BCL2-IGH@ fusion - matc
round and oval cells growing singly or in small clumps in suspension
Mycoplasma: contamination was eliminated with Plasmocin, then negative in microbiological culture, PCR assays
Immunology: CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 -, CD 138 -, HLA-DR +, sm/cyIgG +, sm/cyIgM -, sm/cyka
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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