The growth of WEHI-3 is inhibited by 4 ng/ml LPS and blocked by higher concentrations. Dextran sulfate at 30 to 40 ng/ml also inhibits growth. Latex beads are phagocytized but are not toxic. Zymosan and BCG are phagocytized and block growth. The cells exhibit only weak effector activity in antibody dependent cell mediated cytotoxicity.
Mus musculus (mouse)
Murine hypotetraploid karyotype with 19% polyploidy - 73/74(70-79)<4n> - 2-3 centric fusion markers present.
Blood, peripheral; leukemia
small, round, single cells growing adherently and partly in suspension
Fluorescence (DAPI) test: negative; Mycoplasma specific PCR: negative; Bacteria
specific PCR: negative
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 4 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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