This is one of a number of cell lines derived from malignant gliomas which have been isolated by J. Ponten and associates from 1966 to 1969.
Species: human, Caucasian female 44 years old
Homo sapiens ,human
STR DNA Profile
Penta E: 7,14
Penta D: 9,14
The stemline chromosome number is hypodiploid, with the modal chromosome number of 44 occurring in 48% of the cells. The rate of higher ploidity was 5.9%. The following twelve markers were common to all cells, including der(1)t(1;3) (p22;q21) der(16)t(1;16) (p22;p12), del(9) (p13) and nine others. The marker der(1) had two copies in most cells. All S metaphases were monosomic for the X chromosome. Neither homogeneously staining regions (HSR) nor double minutes (DM) were detected, as was the case with N1, N6 and N9.
Sterility: mycoplasma negative, HOECHST and PCR
Storage and Shipping
Frozen with Culture medium + 50% FBS + 10% DMSO; ship in dry ice; store in liquid nitrogen
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