Established from the peripheral blood of a 53-year-old man with IgE-secreting myeloma (refractory, terminal) in 1968; cells were described to produce IgE lambda; possible fusion partner for hybridoma production; cells express mRNA for BCL2 gene
Human complex hypodiploid karyotype with 6.5% polyploidy - 44(40-46)<2n>XY, -8, -10, -13, -15, +2mar, t(1;11)(p33;q13), add(3)(q27), t(4;11)(q?21;q23), add(7)(q32), add(8)(q24), add(9)(q34), add(10)(p14), add(14)(p11), add(17)(p11), add(18)(p12), der(22)t
round to polygonal, single or clustered cells in suspension, some loosely adherent
Mycoplasma: contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, PCR assays
Immunology: CD3 -, CD5 -, CD10 -, CD13 -, CD19 -, CD20 -, CD34 -, CD37 -, CD38 (+), cyCD79a +, CD138 +, HLA-DR +, sm/cyIgG -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 6 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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