J. Ponten and E. Saksela derived this line (originally 2T) in 1964 from a moderately differentiated sarcoma of the tibia of a 15 year old girl. Viruses were not detected during co-cultivation with WI-38 cells or in CF tests against SV40, RSV or adenoviruses. The cell line expresses osteosacoma derived growth factor (ODGF), Blood Type A; Rh+; HLA A2, Aw30, B12, Bw35, B40(+/-).
Homo sapiens, human
STR DNA Profile
Penta E: 10,13
Penta D: 9
(P11-46) hypodiploid to near tetraploid; (P111-118) modal numbers 34 to 37 and 64 to 67 with abnormalities including dicentrics,breaks, rings, and pulverizations plus acrocentric subtelocentric and minute markers.
The U-2 OS cell line can be used as a suitable transfection host.
Tests for mycoplasma, bacteria and fungi were negative
Storage and Shipping
liquid nitrogen vapor phase
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