This is one of a number of cell lines derived from malignant gliomas (see also U-87 MG, U-138 MG and U-373 MG) by J. Ponten and associates from 1966 to 1969.
Homo sapiens ,human
STR DNA Profile
Penta E: 7
Penta D: 13
The line has a near pentaploid chromosome number and a wide range of chromosome number distribution (40% of the cells had numbers ranging from 110 to 115). The following 14 markers were found in most metaphases: t(1p,2p), t(3p,?), t(4p,11q), t(7p,22q), M6, t(9q,?), i(11q)18q t(10q,?), M14, M15, M16, M17 and t(10q,22q); 6 of these were found in some and 10 were seen in one only. Normal chromosomes 7, 8, 12, 19, 20 and 22 had 5 to 6 copies per cell; the X had two copies and the Y was absent.
DMEM supplemented with 4.5g/L glucose, 2mM L-glutamine and 10% h.i.FBS
or SuperCult® U-118 MG medium
Tests for mycoplasma, bacteria and fungi were negative
Storage and Shipping
liquid nitrogen vapor phase
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