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TE-671

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Cat.No.
CSC-C0362
Description
The original TE671 cell line was initiated by explant culture from biopsy fragments of a cerebellar medulloblastoma. The cell strain was isolated by picking a few of the multilayered round or polygonal cells which were interspersed in monolayers of stromal glial cells using Pasteur pipettes. Forms colonies in soft agar medium. Neural or glial elements were not observed upon examination with the electron microscope and no viruses were detected. The cells possess functional nicotinic acetylcholine receptors. No choline acetyltransferase or tyrosine hydroxylase activities are detectable. Cytogenetic studies by Stratton et al. (1989) have revealed that TE671 and RD are derivatives of the same cell line of rhabdomyosarcoma origin.
Organism
Homo sapiens, human
STR DNA Profile
Amelogenin: X
CSF1PO: 10,11
D13S317: 13
D16S539: 10,11
D5S818: 10,11
D7S820: 8,12
THO1: 9.3,9.3
TPOX: 9
vWA: 18
D3S1358: 15,17
D21S11: 28,29
D18S51: 13
Penta E: 12
Penta D: 11,13
D8S1179: 11,15
FGA: 20,21
Karyotype
2n=47; 42% have 48 chromosomes
Source
rhabdomyosarcoma
Culture Properties
monolayer, adherent
Morphology
epithelial
Recommended Medium
DMEM and 10% h.i.FBS
Quality Control
Fluorescence (DAPI) test: negative; Mycoplasma specific PCR: negative; Bacteria specific PCR: negative
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 10 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Disease
Embryonal rhabdomyosarcoma
Application
This cell line can be used in tumourigenicity studies.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
CSC-6258W
CSC-6266W
CSC-C0367
CSC-C0473
CSC-C0481
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