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T47D;T-47D

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Cat.No.
CSC-C2512
Description
Established from pleural effusion of a ductal carcinoma of the breast of a 54-year-old female. The differentiated epithelial sub-strain T-47D reportedly contains cytoplasmic junctions, receptors to 17-beta-estradiol, other steroids and calcitonin. It will form colonies in soft agar. The cells express the WNT7B oncogene.
Organism
Homo sapiens, human
STR DNA Profile
Amelogenin: X,X
CSF1PO:11,13
D13S317:12
D16S539:10
D5S818:12
D7S820:11
TH01:7,6
TPOX:11
vWA:14
D3S1358: 15,17
D21S11: 28,31
D18S51: 17
Penta E: 7,14
Penta D: 10,12
D8S1179: 13
FGA: 23
Karyotype
This is a hypotriploid human cell line. The modal chromosome number is 65 occurring at 50% and polyploidy at 0.8%. 18 marker chromosomes are common to most cells, of which 7 are paired and 11 are single-copied. The t(8q14q), t(9q17q), t(10q17p) are among 7 paired markers common to most cells. N7, N9, and N10 are absent and N11 is generally present in 4 copies. DM's occurred, but infrequently. Q-band examination did not show the presence of a Y chromosome.
Source
Breast; mammary gland
Culture Properties
monolayer
Morphology
Epithelial
Quality Control
Sterility: mycoplasma contamination eliminated with BM-cyclin; negative, HOECHST and PCR
Storage and Shipping
Frozen with Culture medium + 50% FBS + 10% DMSO; ship in dry ice; store in liquid nitrogen
Disease
Invasive ductal carcinoma
Application
This cell line can be used as a suitable transfection host.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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