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T24;T-24

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Cat.No.
CSC-C0424
Description
Established from the primary tumor of an 81-year-old Caucasian woman with urinary bladder carcinoma (transitional cell carcinoma, grade III) in 1970; described to produce a variety of cytokines (e.g. G-CSF, IL-6 and SCF) and to carry a p53 mutation. Leukocytes and sera from patients with transitional cell carcinoma were cytotoxic to T24 and related lines. T24 cells have a generation time of 19 hours.
Organism
Homo sapiens ,human
STR DNA Profile
Amelogenin: X,X
CSF1PO: 10,12
D13S317: 12,12
D16S539: 9,9
D5S818: 10,12
D7S820: 10,11
THO1: 6,6
TPOX: 8,11
vWA: 17,19
D3S1358: 16,21
D21S11: 29,29
D18S51: 16,18
Penta E: 7,10
Penta D: 11,15
D8S1179: 9,14
FGA: 17,22
Karyotype
hypodiploidy to hypopentaploidy; stemline 86; 2 to 4 telocentrics; 3 to 4 minutes, hypotetraploid to hypertetraploid with abnormalities including dicentrics, breaks, pulverization, minutes and telocentric markers.
Source
bladder, urinary
Culture Properties
monolayer
Morphology
epithelial
Recommended Medium
Quality Control
Mycoplasma: contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, desmin -,
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 2.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
CSC-C0268
CSC-C0334
CSC-C0335
CSC-C0336
CSC-C0387
CSC-C0413
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