Established from a lung metastasis of colon carcinoma in a 72 year old male. Tumour tissue was inoculated sub-cutaneously and serially transplanted in BALB/c nude mice and subsequently established in in vitro culture. The histological characteristics of the tumour were maintained throughout the transplantation procedure. They have receptors for a range of peptide hormones and neurotransmitters. The cells grow as monolayers, exhibit tight junctions and desmosomes between adjacent cells. The cells should be maintained at high density (at least 1/4 confluency) and are positive for keratin by immunoperoxidase staining.
Homo sapiens, human
STR DNA Profile
Penta E: 14
Penta D: 9
The stemline modal chromosome number is 56, occurring at 28% with polyploidy at 12.4%. Eighteen markers are common to most metaphases examined. Normal X and chromosome 13 were absent; chromosomes 2, 4 and 22 were single-copied, and chromosome 12 was 4-copied. No Y chromosome was detected by Q band observation. DM occurred in nearly 50% of the cells.
Carcinoma, colorectal; Colon; from metastatic site: lung
Tests for mycoplasma, bacteria and fungi were negative
Storage and Shipping
liquid nitrogen vapor phase
The T84 cell line can be used for tumourigenicity, neurotransmitter and hormone studies. It is a transplantable human carcinoma cell line and also can be used as a suitable transfection host.
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