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T-84

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Cat.No.
CSC-C0257
Description
Established from a lung metastasis of colon carcinoma in a 72 year old male. Tumour tissue was inoculated sub-cutaneously and serially transplanted in BALB/c nude mice and subsequently established in in vitro culture. The histological characteristics of the tumour were maintained throughout the transplantation procedure. They have receptors for a range of peptide hormones and neurotransmitters. The cells grow as monolayers, exhibit tight junctions and desmosomes between adjacent cells. The cells should be maintained at high density (at least 1/4 confluency) and are positive for keratin by immunoperoxidase staining.
Organism
Homo sapiens, human
STR DNA Profile
Amelogenin: X
CSF1PO: 10
D13S317: 9
D16S539: 10,11
D5S818: 12
D7S820: 8,10
THO1: 6,9
TPOX: 8
vWA: 17,18
D3S1358: 19
D21S11: 31
D18S51: 17
Penta E: 14
Penta D: 9
D8S1179: 15
FGA: 24
Karyotype
The stemline modal chromosome number is 56, occurring at 28% with polyploidy at 12.4%. Eighteen markers are common to most metaphases examined. Normal X and chromosome 13 were absent; chromosomes 2, 4 and 22 were single-copied, and chromosome 12 was 4-copied. No Y chromosome was detected by Q band observation. DM occurred in nearly 50% of the cells.
Source
Carcinoma, colorectal; Colon; from metastatic site: lung
Culture Properties
Adherent
Morphology
epithelial
Recommended Medium
Quality Control
Tests for mycoplasma, bacteria and fungi were negative
Storage and Shipping
liquid nitrogen vapor phase
Application
The T84 cell line can be used for tumourigenicity, neurotransmitter and hormone studies. It is a transplantable human carcinoma cell line and also can be used as a suitable transfection host.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
CSC-6317W
CSC-C0204
CSC-C0253
CSC-C0256
CSC-C0300
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