RPMI-1640 is a modification of McCoy's 5A medium that contains a higher concentration of inositol. The medium is commonly used with mammalian myeloma and hybridoma cells. This formulation does not contain phenol red or L-glutamine.
Supplement with 2 mM L-glutamine prior to use. Modified to contain 10 mM HEPES, 1 mM sodium pyruvate, 4.5 g/L D-glucose, and 1.5 g/L sodium bicarbonate. Note: This reduced level of sodium bicarbonate is intended for use in a 5% CO2 in air environment. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.
Sterility tests are performed on each lot of medium using current USP methods. Each lot of medium is also tested for the ability to support the growth of several different cell lines using both sequential subcultures and plating efficiencies. pH, osmolali
Storage and Shipping
Store medium at 2 °C to 8 °C in the dark when not in use.
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