The kit contains components that when added to the fibroblst basal medium create a complete serum-free culture environment for fibroblasts. The final concentration for each component is as follows:
HLL Supplement: HSA 500 μg/mL, linoleic acid 0.6 mM, lecithin 0.6 μg/mL
L-Glutamine: 7.5 mM
rh FGF: 5 ng/mL
rh EGF/TGF-1 Supplements: 5 ng/mL and 30 pg/mL, respectively
rh Insulin: 5 μg/mL
Hydrocortisone: 1 μg/mL
Ascorbic acid: 50 μg/mL
Sterility Tests: Negative for bacteria, fungi, and yeast.
Functional Tests: Rate of proliferation and morphology of fibroblasts.
A Certificate of Analysis (COA) is available upon request for each lot of Keratinocyte Growth Kit.
Storage and Shipping
-20 °C (or -70 °C for long-term storage)
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