The line was formed by fusing Balb/c spleen cells (from mouse immunized with sheep RBCs) with the P3X63Ag8 myeloma cell line. The cells do not secrete immunoglobulin, are resistant to 8-azaguanine at 20 µg/ml and are HAT sensitive. Sp2/0-Ag14 cells can be used as fusion partners for B cells in the production of hybridomas.
Fluorescence (DAPI) test: negative; Mycoplasma specific PCR: negative; Bacteria
specific PCR: negative
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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