Established from the adenocarcinoma (grade III) of a 51-year-old Caucasian woman. CSAp negative (CSAp-); colon antigen 3, positive; the cells are positive for keratin by immunoperoxidase staining.
Tumorigenecity:yes, in nude mice
Histopathology:adenocarcinoma; grade III
human - female, 51 years old, Caucasian
STR DNA Profile
Human hypotriploid karyotype with 12% polyploidy - 62(58-70)<3n>XX/XXX, +2, +2, -8, -9, -10, -10, -12, -13, -14, -18, -19, -22, +mar, der(1)t(1;1)(p21;q22), del(4)(q21), add(5)(p15), del(6)(q24-q25), i(8q), del(9)(p21), i(9q), add(13)(p13), add(14)(p13).
Mycoplasma: contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 2.5-3.0 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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