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SW-480

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Cat.No.
CSC-C0256
Description
Established from the tumor of a 50-year-old Caucasian man with colon adenocarcinoma (grade 4, Duke class B). The cells express elevated levels of p53 protein. The cells have been reported to produce GM-CSF. SW-480 were described to produce carcinoembryonic antigen (CEA) 0.7 ng/10 6 cells/10 days; keratin; transforming growth factor beta, myc +; myb +; ras +; fos +; sis +; p53 +; abl -; ros -; src -, HLA A2, B8, B17; blood type A; Rh+. The cells are positive for keratin by immunoperoxidase staining and are positive for expression of c-myc, K-ras, H-ras, N-ras, myb, sis and fos oncogenes.
Organism
Homo sapiens, human
STR DNA Profile
Amelogenin: X,X
CSF1PO: 13,14
D13S317: 12
D16S539: 13
D5S818: 13
D7S820: 8
THO1: 8
TPOX: 11
vWA: 16
D3S1358: 15
D21S11: 30,30.2
D18S51: 13
Penta E: 10
Penta D: 9,15
D8S1179: 13
FGA: 24
Karyotype
The stemline chromosome number is hypotriploid and 11-12 marker chromosomes were common. Both double minutes and dicentrics were observed in 8% of each metaphase examined.
Source
Colon
Culture Properties
monolayer, adherent
Morphology
Epithelial
Recommended Medium
SuperCult® SW-480 Medium
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 3 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Disease
Dukes' type B, colorectal adenocarcinoma
Application
The SW-480 cell line can be used as a suitable transfection host. This cell line has a mutation in codon 12 of the ras proto-oncogene, and can be used as a positive control for PCR assays of mutation in this codon.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
CSC-6317W
CSC-C0204
CSC-C0253
CSC-C0300
CSC-C0392
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