Isolated from colorectal adenocarcinoma tissue. The cells are CSAp negative (CSAp-) and colon antigen 3 negative. They are positive for keratin by immunoperoxidase staining. The line is positive for expression of c-myc, K-ras, H-ras, myb, sis and fos oncogenes. N-myc and N-ras expression were not detected. Tumor specific nuclear matrix proteins CC-4, CC-5 and CC-6 are expressed.
Homo sapiens ,human
STR DNA Profile
Penta E: 10,12
Penta D: 11,13
Colon; grade III; Dukes type C
Tested negative for mycoplasma by: DAPI, MycoAlert and PCR.
Storage and Shipping
Frozen with 52.5% RPMI-1640, 40% FBS, 7.5% DMSO at about 4-5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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