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SKNO-1

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Cat.No.
CSC-C0671
Description
Established in 1990 from the bone marrow of a 22-year-old man with acute myeloid leukemia (AML M2) in second relapse
Organism
Human
Karyotype
Clonally heterogeneous human near-diploid karyotype; 46(42-48)<2n>X, -X, +5, +6, +16, add(2)(q22), i(5)(p10), add(6)(q27) or i(6)(p10), der(7)t(7;9)(q35.2;q21), t(8;21)(q22;q22), ider(9)(p10)add(9)(p21), add(11)(p15) or inv(11)(p15q24), der(16;17)(q10;q10
Source
acute myeloid leukemia
Culture Properties
suspension
Morphology
round to polymorphic cells growing singly or occasionally in small clusters in suspension
Recommended Medium
90% RPMI-1640 + 10% h.i. FBS + 10 ng/ml GM-CSF
Quality Control
Mycoplasma: negative in microbiological culture, PCR assays
Immunology: CD3 -, CD4 +, CD13 +, CD14 -, CD15 -, CD19 -, HLA-DR +
Viruses: PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/-II -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 10 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.

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