This cell line has been established by J.L. Biedler in 1971. It has moderate dopamine-beta-hydroxylase activity as well as formaldehyde induced fluorescence indicative of intracellular catecholamines. This cell line was found to have moderate dopamine - beta - hydroxylase activity as well as formaldehyde induced fluorescence indicative of intracellular catecholamines.
Homo sapiens, human
STR DNA Profile
Amelogenin: X, X
TPOX: 9, 11
vWA: 17, 18
D3S1358: 15, 15
D21S11: 30, 31.2
D18S51: 13, 14
Penta E: 16, 18
Penta D: 9, 13
D8S1179: 10, 10
FGA: 21, 25
Hypodiploidy to pseudodiploidy. Abnormalities including double minutes, breaks; large submetacentric, telocentric and small telocentric markers (originator). (P32) Hypodiploid to hyperdiploid and triploid to hypotetraploid with abnormalities including dicentrics, breaks, double minutes (DM), large subtelocentric and small telocentric chromosomes.
Brain; from metastatic site: supra-orbital area
Tests for mycoplasma, bacteria and fungi were negative
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6cells/ampoule; ship in dry ice; store in liquid nitrogen
The SK-N-MC cell line can be used as a suitable transfection host.
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