Tumorigenecity: Yes, in nude mice; forms malignant melanoma
Histopathology: malignant melanoma; skin; from metastatic site: axillary node
Subculture: Remove medium, add fresh 0.25% trypsin solution for 1 minute, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.
Split ratio: 1:3 to 1:6
Media change: 2 to 3 times per week
Reverse transcritase: Production
Homo sapiens (human)
STR DNA Profile
modal number = 120; range = 95 to 133.
Tests for mycoplasma, bacteria and fungi were negative
Storage and Shipping
Frozen with 52.5% RPMI-1640, 40% FBS, 7.5% DMSO at about 4-5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
SK-MEL-5 is a suitable transfection host. This line also serves as a source of target cells for the detection of melanoma specific antibody in patients with the disease.
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