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SK-MEL-5

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Cat.No.
CSC-C9606L
Description
Tumorigenecity: Yes, in nude mice; forms malignant melanoma
Isoenzyme: PGM1,1-2;PGM3,1;ES-D,1;AK-1,1;GLO-1,1-2;G6PD,B
Histopathology: malignant melanoma; skin; from metastatic site: axillary node
Subculture: Remove medium, add fresh 0.25% trypsin solution for 1 minute, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.
Split ratio: 1:3 to 1:6
Media change: 2 to 3 times per week
Reverse transcritase: Production
Tissue of Origin
Skin
Organism
Homo sapiens (human)
STR DNA Profile

Amelogenin: X
CSF1PO: 10,13
D13S317: 10,12
D16S539: 10,12
D5S818: 11,13
D7S820: 9,12
THO1: 6,9
TPOX: 11
vWA: 14,18
Karyotype
modal number = 120; range = 95 to 133.
Culture Properties
adherent
Morphology
Stellate
Recommended Medium
EMEM and 10% h.i. FBS
Quality Control
Tests for mycoplasma, bacteria and fungi were negative
Storage and Shipping
Frozen with 52.5% RPMI-1640, 40% FBS, 7.5% DMSO at about 4-5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Disease
Cutaneous melanoma
Application
SK-MEL-5 is a suitable transfection host. This line also serves as a source of target cells for the detection of melanoma specific antibody in patients with the disease.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
Customer Support & Price Inquiry
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CONTACT US USA
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Tel: 1-631-626-9181
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