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SK-LU-1

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Cat.No.
CSC-C8940H
Description
Tumorigenecity:Yes, in immunotolerant rats
Isoenzyme:Me-2,1;PGM3,1;PGM1,2;ES-D,1;AK-1,1;GLO-1,2;G6PD,B
Histopathology:adenocarcinoma
Subculture:Remove medium, add with fresh 0.25% trypsin solution for 1 min, remove trypsin and let the culture sit at room temperature for 5 to 10 mins. Add fresh medium, aspirate and dispense into new flasks.
Subculture every 6 to 8 days
Media change:twice per week
Reverse transcritase:Production
Tissue of Origin
Lung
Organism
Homo sapiens (human)
STR DNA Profile
Amelogenin: X,X
CSF1PO: 10
D13S317: 10
D16S539: 8
D5S818: 11
D7S820: 9
THO1: 7
TPOX: 8,10
vWA: 16,17
D3S1358: 18
D21S11: 29,30.2
D18S51: 18
Penta E: 5
Penta D: 10,13
D8S1179: 10
FGA: 21,22
Karyotype
The stemline chromosome number is hypotetraploid, with the 2S component occurring at 4.4%. Marker chromosomes 1p, t(1q;11q); 11q+; t(13;?); 16q+; t(12q; 18q); M10; t(2q;13q); i(15); and ?t(xp;21q) occurred in all S metaphases, and t(1p;?); t(1p;14q); t(16;?), and t(14;21) occurred in some. In addition, 4 to 9 small markers of unidentifiable origin occurred frequently. Chromosome No. 7 was generally hexasomic, X chromosomes were disomic, and normal No. 15 was absent. No Y chromosome was detected in the QM stained preparation.
Morphology
epithelial
Recommended Medium
EMEM and 10% h.i. FBS
Quality Control
Tests for mycoplasma, bacteria and fungi were negative
Storage and Shipping
liquid nitrogen vapor phase
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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