Tumorigenecity:Yes, in immunotolerant rats
Subculture:Remove medium, add with fresh 0.25% trypsin solution for 1 min, remove trypsin and let the culture sit at room temperature for 5 to 10 mins. Add fresh medium, aspirate and dispense into new flasks.
Subculture every 6 to 8 days
Media change:twice per week
Homo sapiens (human)
STR DNA Profile
Penta E: 5
Penta D: 10,13
The stemline chromosome number is hypotetraploid, with the 2S component occurring at 4.4%. Marker chromosomes 1p, t(1q;11q); 11q+; t(13;?); 16q+; t(12q; 18q); M10; t(2q;13q); i(15); and ?t(xp;21q) occurred in all S metaphases, and t(1p;?); t(1p;14q); t(16;?), and t(14;21) occurred in some. In addition, 4 to 9 small markers of unidentifiable origin occurred frequently. Chromosome No. 7 was generally hexasomic, X chromosomes were disomic, and normal No. 15 was absent. No Y chromosome was detected in the QM stained preparation.
Tests for mycoplasma, bacteria and fungi were negative
Storage and Shipping
liquid nitrogen vapor phase
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