Tumorigenecity:Yes, in nude mice; forms large cell carcinoma consistent with hepatoma
Subculture:Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37ºC) until the cells detach (about 10 min). Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.
Split ratio:1:2 to 1:4
Media change:2 to 3 times weekly
Reverse transcritase: Production
Homo sapiens (human)
Endothelial (Heffelfinger, 1992)
STR DNA Profile
(P11) hyperdiploid to hypotriploid (+A3, +C, +E, +F, +G, -A, -D) with abnormalities including dicentrics, acrocentric fragments, secondary constrictions, pulverizations, and large subtelocentric and submetacentric markers.
Tests for mycoplasma, bacteria and fungi were negative
Storage and Shipping
liquid nitrogen vapor phase
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