Description
SH-SY5Y is one of three serially isolated neuroblast clones (SH-SY, SH-SY5, SH-SY5Y) of the human neuroblastoma cell line SK-N-SH which was established in 1970 from a metastatic bone tumor. The cells exhibit moderate levels of dopamine beta hydroxylase activity. They can convert glutamate to the neurotransmitter GABA. SH-SY5Y cells have a reported saturation density greater than 1 x 10^6 cells/cm2. The loss of neuronal characteristics has been described with increasing passage numbers (approx. passage 20). Neuronal markers or uptake of noradrenalin should be determined routinely. It is recommended not to use at passage numbers higher than 20 unless neuronal characteristics are controlled.
Organism
Homo sapiens, human
Source
Metastatic site: bone marrow
Recommended Medium
90% MEM:Ham's F12 medium (1:1 mixture) and 10%
FBSCulture Properties
Monolayer
Morphology
The cells grow as clusters of neuroblastic cells with multiple, short, fine cell processes (neurites). Cells will aggregate, form clumps and float; a confluent monolayer is not formed.
STR DNA Profile
Amelogenin: X
CSF1PO: 11
D13S317: 11
D16S539: 8,13
D5S818: 12
D7S820: 7,10
THO1: 7,10
TPOX: 8,11
vWA: 14,18
D3S1358: 15,16
D21S11: 31,31.2
D18S51: 13,16
Penta E: 7,11
Penta D: 10,12
D8S1179: 15
FGA: 23.2,24
Karyotype
modal number = 47; the cells possess a unique marker comprised of a chromosome 1 with a complex insertion of an additional copy of a 1q segment into the long arm, resulting in trisomy of 1q.
Applications
The AGS cell line can be used for neurotransmitter studies , it can also be used as a suitable transfection host.
Quality Control
Tests for mycoplasma, bacteria and fungi were negative
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
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