SH-SY5Y is one of three serially isolated neuroblast clones (SH-SY, SH-SY5, SH-SY5Y) of the human neuroblastoma cell line SK-N-SH which was established in 1970 from a metastatic bone tumor. The cells exhibit moderate levels of dopamine beta hydroxylase activity. They can convert glutamate to the neurotransmitter GABA. SH-SY5Y cells have a reported saturation density greater than 1 x 10^6 cells/cm2. The loss of neuronal characteristics has been described with increasing passage numbers (approx. passage 20). Neuronal markers or uptake of noradrenalin should be determined routinely. It is recommended not to use at passage numbers higher than 20 unless neuronal characteristics are controlled.
Homo sapiens, human
Metastatic site: bone marrow
DMEM supplemented with 4.5g/L glucose, 2mM L-glutamine and 10% h.i.FBS
or SuperCult® SH-SY5Y medium
The cells grow as clusters of neuroblastic cells with multiple, short, fine cell processes (neurites). Cells will aggregate, form clumps and float; a confluent monolayer is not formed.
STR DNA Profile
Penta E: 7,11
Penta D: 10,12
modal number = 47; the cells possess a unique marker comprised of a chromosome 1 with a complex insertion of an additional copy of a 1q segment into the long arm, resulting in trisomy of 1q.
The AGS cell line can be used for neurotransmitter studies , it can also be used as a suitable transfection host.
Tests for mycoplasma, bacteria and fungi were negative
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6cells/ampoule; ship in dry ice; store in liquid nitrogen
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