Established from a recurrent well-differentiated transitional papillary tumor of the urinary bladder (clinical stage T2, histological grade G1, treated with gold grains 2 years earlier and diathermy after recurrence 10 months later) of a 63-year-old man in 1968.
Homo sapiens, human
STR DNA Profile
(P174) hyperdiploid and hypotetraploid to hypertetraploid with abnormalities including dicentrics, breaks, translocations and minutes.
transitional cell papilloma; bladder, urinary
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM -, GFAP -, neurofilament -,
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 2-3 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
The RT-4 cell line can be used for tumourigenicity studies and can also be used as a suitable transfection host.
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