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RPMI 8226;RPMI-8226

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Cat.No.
CSC-C0447
Description
Established from the peripheral blood of a 61-year-old man with multiple myeloma (IgG lambda-type) at diagnosis in 1966; described to produce and secrete only lambda light chains (but not heavy chains). There is no evidence of heavy chain production (cytoplasmic or secreted).
Tissue of Origin
Peripheral Blood
Organism
Homo sapiens (human)
STR DNA Profile
D3S1358: 16,17
vWA: 16,18
FGA: 19
Amelogenin: X,Y
TH01: 8
TPOX: 8,11
CSF1P0: 12
D5S818: 11,13
D13S317: 11
D7S820: 9,10
Karyotype
Human flat-moded hypotriploid karyotype with 7.5% polyploidy - 62-67<3n>XXY, -1, +5, +6, +7, -8, -9, -10, +11, -12, -13, -14, +16, +18, -19, +21, -22, der(1)t(1;1)(p36.3;q11-12), add(3)(q27-29)x2, del(4)(p14p15.4), der(5;6)(q10;p10)x1-2, del(5)(q32).
Culture Properties
suspension
Morphology
lymphoblast
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD5 -, CD10 -, CD13 -, CD14 (+), CD19 -, CD20 -, CD21 (+), CD34 -, CD38 +, CD80 -, CD138 +, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 3 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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