ASYMPTOMATIC OR UNDIAGNOSED AND GENETICALLY RELATED TO AN AFFECTED INDIVIDUAL;
Cells were cultured in DMEM/F12 supplemented with 20% KOSR, 2 mM L-glutamine, 0.1 mM Non-essential amino acids, 0.1 mM 2-mercaptoethanol and bFGF (concentration stated on Certificate of Analysis) and plated on gelatin coated plates using irradiated CF-1 mouse embryonic fibroblasts as feeder layer.
Parkinson Disease hiPSC samples expanded and characterized in-house;
Age: 58 YR
The cell line submitted to the Repository was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation. Steady-state mRNA
Storage and Shipping
Liquid nitrogen. Dry ice.
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